Increasing evidence suggests that the persistent herpesvirus cytomegalovirus (CMV) plays an important role in human immunosenescence, meriting special attention in studies designed to understand stress, immune function, and health. Age-associated alterations in systemic immunity, referred to as 'immunosenescence', are thought to contribute to the increased incidence and severity of infectious disease in older persons, as well as decreased response to vaccination. Immune system decline is one of the hallmarks of aging. The validity of a DBS assay for CMV antibodies will enable its inclusion in population-based surveys and other studies collecting DBS samples in non-clinical settings, increasing scientific understanding of the interaction of social and biological stress and immune function. 96), and precision, reliability, and linearity of the DBS assay were within acceptable ranges. Correlation between DBS and plasma values was linear and high (Pearson correlation R =. The method was evaluated through analysis of precision, reliability, linearity, and correlation between matched serum and DBS samples collected from 75 volunteers. ResultsĪnalysis of CMV in dried blood spot samples (DBS) was based on modifications of a commercially available protocol for quantifying CMV antibodies in serum or plasma. This paper describes and validates a minimally invasive method for assessing antibodies against CMV in finger stick whole blood spot samples for use as an indirect marker of an aspect of cell-mediated immunity. Cytomegalovirus (CMV) is a prevalent herpesvirus with links to both stress and aging.
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